Journal of Family Medicine and Primary Care

ORIGINAL ARTICLE
Year
: 2019  |  Volume : 8  |  Issue : 6  |  Page : 2008--2011

Cryptococcal meningitis: An under-reported disease from the hills of Uttarakhand: A hospital-based cross-sectional study


Aroop Mohanty1, Mohit Bhatia1, Ankita Kabi2, Kuhu Chatterjee1, Neelam Kaistha1, Balram Ji Omar1, Puneet K Gupta1, Pratima Gupta1 
1 Department of Microbiology, All India Institute of Medical Sciences, Rishikesh, Uttarakhand, India
2 Department of Emergency Medicine, All India Institute of Medical Sciences, Rishikesh, Uttarakhand, India

Correspondence Address:
Dr. Mohit Bhatia
Department of Microbiology, All India Institute of Medical Sciences, Virbhadra Marg, Rishikesh, Uttarakhand - 249 203
India

Background: Cryptococcal meningitis is a fatal opportunistic neuroinfection and an AIDS defining illness. It can also occur in non-HIV patients who are immunodefecient due to chronic glucocorticoid use, organ transplantation, malignancy and sarcodiosis. Materials and Methods: A cross-sectional study was conducted in a tertiary care hospital from July to December 2018. CSF samples of 364 patients were received by Microbiology laboratory during this period for the purpose of aerobic bacterial, fungal and TB culture, respectively. All samples were subjected to examination by direct wet mount, Gram stain and India ink preparation. Ziehl Neelsen staining, solid culture for Mycobacterium tuberculosis on Lowenstein Jensen medium and Gene Xpert was also performed on all CSF samples. These samples were further subjected to fungal culture on Sabouraud's dextrose agar. Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF-MS) was used for identifying all bacterial (except M. tuberculosis) and fungal isolates. Results: Out of 364 CSF samples received, 288 were sterile after 48 hours of aerobic incubation. Bacterial isolates, M. tuberculosis and Cryptococcus spp. were obtained in culture from 51, 21 and 4 samples, respectively. The prevalence of cryptococcal meningitis in our study was 1.09% (4/364). Cryptococcus neoformans var grubii was the most common isolate (2/4; 50%) followed by Cryptococcus neoformans var neoformans (1/4; 25%) and Cryptococcus neoformans var gattii (1/4; 25%), respectively. Conclusion: Cryptococcal meningitis is a rapidly fatal condition which requires a high index of suspicion and calls for a collective effort from family physicians and diagnosticians alike. This disease is under-reported from Uttarakhand and therefore calls for further research from this region.


How to cite this article:
Mohanty A, Bhatia M, Kabi A, Chatterjee K, Kaistha N, Omar BJ, Gupta PK, Gupta P. Cryptococcal meningitis: An under-reported disease from the hills of Uttarakhand: A hospital-based cross-sectional study.J Family Med Prim Care 2019;8:2008-2011


How to cite this URL:
Mohanty A, Bhatia M, Kabi A, Chatterjee K, Kaistha N, Omar BJ, Gupta PK, Gupta P. Cryptococcal meningitis: An under-reported disease from the hills of Uttarakhand: A hospital-based cross-sectional study. J Family Med Prim Care [serial online] 2019 [cited 2021 Jun 16 ];8:2008-2011
Available from: https://www.jfmpc.com/article.asp?issn=2249-4863;year=2019;volume=8;issue=6;spage=2008;epage=2011;aulast=Mohanty;type=0