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Year : 2021  |  Volume : 10  |  Issue : 2  |  Page : 615-624

Perplexity vs Clarity in choosing the right molecular diagnostic techniques for SARS-COV2 detection in Indian setup

1 Department of Microbiology, LHMC, New Delhi, India
2 Department of Virology, PGIMER, Chandigarh, India
3 Department of Biochemistry, LHMC, New Delhi, India

Correspondence Address:
Dr. Ishani Bora
Assistant Professor, Department of Virology, PGIMER Chandigarh
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/jfmpc.jfmpc_1793_20

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After the havoc created by Spanish flu a century ago, the world is witnessing exactly a similar pandemic situation since the beginning of the year 2020. The unexplained respiratory illness with high morbidity & mortality which started in Wuhan, China and spread across the world was finally termed as COVID-19 disease caused by SARS-CoV-2 and later announced as pandemic by WHO. This novel virus SARS-CoV-2 is a new variant of SARS corona virus with high infectivity and mysterious pathophysiology. The major step towards containment of this pandemic is to scale up the testing for SARS-CoV-2 and thereby isolating and managing the patients at the earliest. Molecular amplification based methods such a Real time Polymerase chain reaction (RT-PCR), CBNAAT and TrueNAT are the most commonly used techniques for detection of SARS-CoV2. To utilize these diagnostic facilities optimally in the management of the suspected COVID 19 patients, it is of utmost importance for the healthcare providers to understand the intricacies related to these technologies. Thus, the technical details along with the pros & cons of these three amplification-based technologies for proper understanding of these diagnostic modalities for SARS COV-2 diagnosis are discussed herewith.

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